Haplobank

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If you found several clones for your gene-of-interest, here is how to decide which one to take:  
  • In clones highlighted in green, homozygous integration sites are confirmed already.
  • Go for blue clones (only one gene trap insertion) rather than grey clones (more than one gene trap insertion).
  • Go for disruptive mutations (1Intron, Intron, 5'UTR, CDS, and ncExon). If possible we would recommend going for an insertion in the first Intron (1Intron). Clones that carry the gene trap in an intergenic region (Upstream, InterDown, InterUp), cannot be regarded as knockouts. Please also keep in mind that a mutation in the coding sequence is phenotypically not reversible!
  • Decide whether or not you want to start with a clone in which the gene trap cassette is inserted in "sense" or "antisense", and is thus disruptive or non-disruptive at the beginning.
  • The choice of the mutagen is then the last thing: Tol2GT/Retro/Lenti are enhanced gene traps while Tol2 is a polyA-trap that carries EGFP!
Cell ID
Gene
Mutation Site of mutagen integration: disruptive (1Intron, Intron, 5'UTR, CDS, and ncExon) or non-disruptive (Upstream, InterDown, InterUp). Move the mouse over each feature for detailed information or see FAQs for priorities regarding several assigned features.
Rel. Orientation Insertion of the gene trap cassette: sense means disruptive, antisense non-disruptive. Gene trap cassettes can be inverted, see protocol section
Mutagen System used to mutagenize the cells. For information about the system and the corresponding sequences go to "Haplobank Resources" or use the provided link of the specific mutagen.
Gen.Location
RPKM Reads Per Kilobase per Million mapped reads: a method to quantify gene expression. RNA sequencing data is normalized for total read length and the number of sequencing reads.
PerfectGT The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame.
Integration sites were identified using three different approaches: two inverse PCRs using the two different restriction enzymes E1 and a barcode PCR, all followed by NGS. If the mapping strategy was successful, it has a green light. Quality Control
AN3-12 wildtype
 
 
 
 
 
No PerfectGT
 
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AL714024.1
ncExon Site of mutagen integration: non-coding exon of a ncRNA.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
12 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
Upstream Site of mutagen integration: region 2000bp upstream of the annotated transcription start site.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
12 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
470 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
28 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
28 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
12 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
12 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
Upstream Site of mutagen integration: region 2000bp upstream of the annotated transcription start site.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
13 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
13 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
29 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
57 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
7SK
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AC157986.1
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Ide
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
48 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
124 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AC119939.1
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AC155640.1
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
35 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
336 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
32 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
SNORA17
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AC122043.1
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
13 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
125 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
70 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
SNORA81
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
U2
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
57 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
57 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
3UTR Site of mutagen integration: 3' UTR of the annotated transcript.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
5UTR Site of mutagen integration: 5' UTR of the annotated transcript
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
AC133083.1
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
30 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
1Intron Site of mutagen integration: first intron of the annotated transcript.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
20 
The insertion site is predicted with high certainty to be disruptive for all RefSeq transcripts of this gene. The splice acceptor is integrated in a genomic locus common to all RefSeq transcripts (intronic and exonic) and before 50% of the longest open reading frame. PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterUp Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Upstream Site of mutagen integration: region 2000bp upstream of the annotated transcription start site.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BG Clone  
InterDown Site of mutagen integration: Intergenic region downstream/upstream. The downstream and upstream region of a gene is the region between the gene of interest and the neighboring gene, which is not covered by one of the other features (1Intron, Intron, 5'UTR, CDS, ncExon, Upstream). Please see FAQs for more information.
antisense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone  
Intron Site of mutagen integration: one of the introns of the annotated transcript, excluding the first intron (which is annotated as 1intron).
sense
Tol2-polyA
Mutagen Tol2-polyA
Click here for sequence
Tol2-polyA 
18 
No PerfectGT
BG Clone iPCR1
BG Clone iPCR2
BG Clone BCPCR
BO Clone